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Species and subspecies of Fusobacterium , genes targeted, and primer and probe sequences used in the qPCR assay.

Journal: Frontiers in Microbiology

Article Title: Longitudinal assessment of the prevalence of Fusobacterium necrophorum , Fusobacterium var ium , and Salmonella enterica in the nasal cavity, ruminal fluid, and feces of finishing beef steers with and without liver abscesses

doi: 10.3389/fmicb.2025.1565303

Figure Lengend Snippet: Species and subspecies of Fusobacterium , genes targeted, and primer and probe sequences used in the qPCR assay.

Article Snippet: Briefly, samples were partially thawed at room temperature to transfer 100 mg of sample into ZR BashingBead™ Lysis Tubes containing 375 μL ZymoBIOMICS™ Lysis Solution, 375 μL of DNA/RNA Shield™, and 1×10 6 copies of lambda phage genome (N3011S; New England Biolabs, Ipswich, MA).

Techniques: Sequencing, Amplification